Evaluation of Inorganic and Organic Bone Components after Application of an Apatite-Coated Al2O3 Implants As Scaffolds For Bone Repair
نویسندگان
چکیده
The aim of this work was to study the influence of uncoated and apatite-coated Al2O3 implants on bone regeneration after 30 days of surgery in New Zealand white rabbits. Twelve samples of Al2O3 implants were prepared and half of them (n = 6) were apatite-coated by the modified biomimetic method. Three experimental groups were tested as Group C – control, surgery procedure without ceramic implant, Group Ce – uncoated Al2O3 implants (n = 6) and Group CeHA apatite-coated Al2O3 implants (n = 6). The mineralization of the Al2O3 implants was analyzed in bone fragments using FT-Raman spectroscopy. Raman peaks at 959 cm and 2940 cm evaluated the inorganic and organic bone content, respectively. In vivo citotoxicity was analyzed using micronucleus test. Inorganic and organic content were higher in CeHA samples than in Ce and C (CeHA > Ce > C). FT-Raman spectroscopy showed that the higher the deposition of the organic matrix, more mineralization occurred. The micronucleus test showed that the uncoated and apatite-coated Al2O3 implants were non-cytotoxic and safe to in vivo applications.
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